Marijuana (Cannabis sativa) has long been known to contain antibacterial cannabinoids, whose potential to address antibiotic resistance has not yet been investigated. All five major cannabinoids (cannabidiol (1b), cannabichromene (2), cannabigerol (3b), ∆9-tetrahydrocannabinol (4b), and cannabinol (5)) showed potent activity against a variety of methicillin-resistant Staphylococcus aureus (MRSA) strains of current clinical relevance. Activity was remarkably tolerant to the nature of the prenyl moiety, to its relative position compared to the n-pentyl moiety (abnormal cannabinoids), and to carboxylation of the resorcinyl moiety (pre-cannabinoids). Conversely, methylation and acetylation of the phenolic hydroxyls, esterification of the carboxylic group of pre-cannabinoids, and introduction of a second prenyl...

The oil of the seeds, petroleum ether and methanol extracts of the whole plant of Cannabis sativa belonging to the family Cannabinaceae were screened for their antimicrobial activity against two Gram positive organisms (Bacillus subtilis, Staphylococcus aureus), two Gram negative organisms (Escherichia coli, Pseudomonas aeruginosa) and two fungi namely Aspergillus niger and Candida albicans using the cup plate agar diffusion method. The oil of the seeds of Cannabis sativa exerted pronounced antibacterial activity (21 - 28 mm) against Bacillus subtilis and Staphylococcus aureus, moderate activity (15 mm) against Escherichia coli and high activity (16 mm) against Pseudomonas aeruginosa and inactive against the two fungi tested. The petroleum ether extract of the whole plant exhibited pronounced antibacterial activity...

The essential oils of five different cultivars of Cannabis sativa contained as main compounds ˛-pinene, myrcene, trans-ˇ-ocimene, ˛-terpinolene, trans-caryophyllene and ˛-humulene. The content of ˛- terpinolene divided the cultivars in two distinct groups, an Eastern European group of cultivars of approximately 8% and a French group of cultivars of around 16%. Therefore, this compound might be suitable as a genetic marker for the two breeding centres for the fibre types of Cannabis sativa. The content of trans-caryophyllene was up to 19%. However, the content of caryophyllene oxide did not exceed 2%. The antimicrobial activity of the essential oil of Cannabis sativa can be regarded...

Abstract Drug resistance of microorganisms is a big threat for the human health. In order to overcome this problem an alternative potential treatment is the use of herbal medicines. Plants are not only considered as dietery supplements to living organisms but also tradionally used for treating many diseases and no sign of drug resistance has been reported till now. The plant leaves were identified as Cannabis sativa L. The cannabinoids were extracted by aqueous extraction found a total yield of 3.8g while as acetone extract 4.8g. The protein content in crude extract of Cannabis sativa L. for aqueous extract was found 112µg/ml and for...

Plant materials are important for animal and human health care and also important for microbial controlling program. This present study has been attempt to determine the antibacterial activity of Cannabis sativa leaf extract to some selective pathogenic bacterial strains such as Staphylococcus aureus, Escherichia coli, Pseudomunas aeruginosa, Enterococcus faecalis, Salmonella typhi and Klebsiella by using leaf ethanol extract and Hot water extract. Antibacterial activity of Cannabis sativa was evaluated by well diffusion methods. The highest zone of inhibition produced by ethanol extract. The leaf of Cannabis Sativa exerted pronounced antibacterial activity (24.1mm) against Staphylococcus aureus, (10.3mm) against Pseudomonas aeruginosa, (22.2mm) against Escherichia coli, (18.1mm)...

Although marijuana is now consumed extensively, little is known of its biologic effects on the lung. To study this problem, the intrapulmonary inactivation of an aerosolized challenge of Staphylococcus aureus was quantified in rats exposed to graded amounts of fresh marijuana smoke. Controls inactivated 85.1 percent +/- 0.3 percent of the bacteria six hours after inoculation. Following an in vivo accumulative exposure to smoke from progressively increasing numbers of marijuana cigarettes for periods of ten minutes each hour for five consecutive hours, intrapulmonary bacterial inactivation was impaired in a dose-dependent manner. Evaluation of the effects of parenterally administered delta-9-tetrahydrocannabinol (THC) or of exposure to...