Plant materials are important for animal and human health care and also important for microbial controlling program. This present study has been attempt to determine the antibacterial activity of Cannabis sativa leaf extract to some selective pathogenic bacterial strains such as Staphylococcus aureus, Escherichia coli, Pseudomunas aeruginosa, Enterococcus faecalis, Salmonella typhi and Klebsiella by using leaf ethanol extract and Hot water extract. Antibacterial activity of Cannabis sativa was evaluated by well diffusion methods. The highest zone of inhibition produced by ethanol extract. The leaf of Cannabis Sativa exerted pronounced antibacterial activity (24.1mm) against Staphylococcus aureus, (10.3mm) against Pseudomonas aeruginosa, (22.2mm) against Escherichia coli, (18.1mm) against Enterococcus faecalis respectively and inactive against the two strains, Salmonella typhi and Klebsiella. The minimum inhibitory effect of C. sativa leaf extract is due to certain compounds present in the C. sativa. Further research should be done to identify the compounds responsible for its activity which can be used as medicines to control a wide range of disease in the world.
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