Please use this link to access this publication: https://www.sciencedirect.com/science/article/abs/pii/S0254629923006804 Abstract Cannabis Sativa L. (Cannabaceae) exerts biological effects through its bioactive compounds. Cannabis Sativa L. essential oil (CSEO) is known to have antibacterial properties that can accelerate wound healing. The present study aimed to evaluate the wound healing efficacy of freshly extracted essential oils from Cannabis Sativa L. hydrodistillated from the leaves of the plant. CSEO was analyzed by gas chromatography-mass spectrometry. Neurobehavioral, histological, and biochemical analyses were performed to examine the effects of CSEO application in an incisional wound model. CSEO demonstrated anti-inflammatory activity by reducing neutrophil levels in blood and skin tissue. CSEO acts by accelerating re-epithelialization and granulation tissue formation. Furthermore, CSEO restored incision-induced neurobehavioral...

Abstract Purpose To examine the prevalence and correlates of sleep problems in a sample of medical cannabis patients. Procedures Adults ages 21 and older (N = 801, M age = 45.8) who were seeking medical cannabis certification (either for the first time or as a renewal) for chronic pain at medical cannabis clinics in southern Michigan completed baseline measures of cannabis use, sleep, pain, and other related constructs. Findings Over half of the sample (59%) met criteria for past 1-month sleep disturbance, defined as at least one sleep problem occurring on 15 or more nights in the past month. Most participants (86%) reported that sleep problems were...

The endocannabinoid system (ECS) regulates multiple physiological processes, including cutaneous cell growth and differentiation. Here, we explored the effects of the major nonpsychotropic phytocannabinoid of Cannabis sativa, (-)-cannabidiol (CBD), on human sebaceous gland function and determined that CBD behaves as a highly effective sebostatic agent. Administration of CBD to cultured human sebocytes and human skin organ culture inhibited the lipogenic actions of various compounds, including arachisebocytesdonic acid and a combination of linoleic acid and testosterone, and suppressed sebocyte proliferation via the activation of transient receptor potential vanilloid-4 (TRPV4) ion channels. Activation of TRPV4 interfered with the prolipogenic ERK1/2 MAPK pathway and resulted in the...

The newly discovered endocannabinoid system (ECS; comprising the endogenous lipid mediators endocannabinoids present in virtually all tissues, their G-protein-coupled cannabinoid receptors, biosynthetic pathways and metabolizing enzymes) has been implicated in multiple regulatory functions both in health and disease. Recent studies have intriguingly suggested the existence of a functional ECS in the skin and implicated it in various biological processes (e.g. proliferation, growth, differentiation, apoptosis and cytokine, mediator or hormone production of various cell types of the skin and appendages, such as the hair follicle and sebaceous gland). It seems that the main physiological function of the cutaneous ECS is to constitutively control the proper...